Phylogenetic Analysis of Bengkulu Citrus Based on DNA Sequencing Enhanced Chemistry Students' System Thinking Skills: Literature Review with Bibliometrics and Experiments

Authors

  • Nadia Amida Universitas Pendidikan Indonesia Author
  • N. Nahadi Universitas Pendidikan Indonesia Author
  • Florentina Maria Titin Supriyanti Universitas Pendidikan Indonesia Author
  • L. Liliasari Universitas Pendidikan Indonesia Author
  • Devri Maulana Institut Teknologi Bandung Author
  • Rendi Zulni Ekaputri Universitas Pendidikan Indonesia Author
  • Indri Sari Utami Universitas Pendidikan Indonesia Author

Keywords:

Citrus, DNA isolation, Phylogenetic analysis, Sequence DNA, System thinking skills

Abstract

The study aims to enhance students' system thinking skills through phylogenetic analysis based on DNA sequencing results. The study is carried out through three steps: (i) pretest: evaluate students' knowledge, (ii) treatment: analysis of affinity relationships through phylogenetics using DNA sequence data, and (iii) postest: measurement of improvement in systems thinking skills after implementation. Evaluations are conducted using written tests as well as worksheets and supported by surveys, questionnaires, and interviews for qualitative analysis. The experiment began with the isolation of citrus DNA, determination of concentration and purity, amplification, reading of sequencing results, and phylogenetics. The results of the teaching analysis show that there is a significant improvement in students' mastery of conceptual and system thinking skills, and there is a high correlation between the pretest and posttest. The problem analysis of interviews and questionnaires is also done to see the enthusiasm for activities as well as the effectiveness of the worksheets used. The study comes with new information on determining the relationship between local citrus in Bengkulu and improving the students' system thinking skills.

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Author Biographies

  • Nadia Amida, Universitas Pendidikan Indonesia

    Universitas Bengkulu

  • Rendi Zulni Ekaputri, Universitas Pendidikan Indonesia

    Universitas Bengkulu

  • Indri Sari Utami, Universitas Pendidikan Indonesia

    Universitas Sultan Ageng Tirtayasa

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Published

2024-08-11

How to Cite

Phylogenetic Analysis of Bengkulu Citrus Based on DNA Sequencing Enhanced Chemistry Students’ System Thinking Skills: Literature Review with Bibliometrics and Experiments. (2024). Indonesian Journal of Science and Technology, 9(2), 337-354. https://ejournal.kjpupi.id/index.php/ijost/article/view/357